Charles DUYCKAERTS - BIOBANQUES May 19, 2016
Biobanking National Infrastructure Meeting
Quality matters: Improving the quality of biological resources
Session 4: Quality control in Biobanks
Quality control in a Brain Bank
Brain often suffers much in the peri-mortem period. Markers of the quality of the tissue have been proposed: the pH of the cerebrospinal fluid has been considered one of those but its use remains limited. As for other tissues, the quality of the RNA may be tested by RIN. Western blots may help to analyze the integrity of the protein. However, one crucial question in neuropathology is the topography of the sample: for the cortex, for instance, the quantity of white matter present in the sample may drastically modify the quantity e.g. of cholesterol. The concentration in neuromediators is highly dependent on the region which is sampled. The distribution of the lesions is also discrete and highly regionalized: the examples of Alzheimer and Parkinson diseases are particularly illustrative. The control of the topography of the sample is thus one of the major difficulties in neuropathological research and has been variously solved. The expertise in neuroanatomy of the person performing the sample is a prerequisite that should be ascertained. The morphological examination of the sample before its use is certainly the safest technique.
WEDNESDAY MAY 18, 2016