Jens BJÖRKMAN - BIOBANQUES May 18, 2016
Biobanking National Infrastructure Meeting
Quality matters: Improving the quality of biological resources
Session 3: Quality control in Genomics / Session 3A
Quality control for the quantification of gene expression biomarkers
Measuring an RT-qPCR signal with an assay targeting a transcript of interest is easy and the data generated often look good, but it is a different story if the signal truly reflects the amount of targeted transcript that actually were present in the sample in vivo. This has to be validated to greatest extent possible by performing relevant controls. I will describe quality control measures to test for degradation of RNA, inhibition, genomic DNA background and also provide means to compensate for interplate variation.
WEDNESDAY MAY 18, 2016