Jens BJÖRKMAN - BIOBANQUES 18 mai 2016


Biobanking National Infrastructure Meeting

Quality matters: Improving the quality of biological resources

Session 3: Quality control in Genomics / Session 3A


Quality control for the quantification of gene expression biomarkers

Measuring an RT-qPCR signal with an assay targeting a transcript of interest is easy and the data generated often look good, but it is a different story if the signal truly reflects the amount of targeted transcript that actually were present in the sample in vivo. This has to be validated to greatest extent possible by performing relevant controls. I will describe quality control measures to test for degradation of RNA, inhibition, genomic DNA background and also provide means to compensate for interplate variation.


  • Fichier manquant Paul Hofman 36
  • Type : Colloques et Séminaires 349
  • Discipline(s) :
  • Durée : 00:31:20
  • Date de mise en ligne : 18 mai 2016
  • Contributeur.trice(s) :
    • COLL Estelle
    • FERNANDEZ David
    • RAMON Olivier
  • Nombre de vues : 185

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