Dust mite allergens in the human GI tract: a potential contributor to intestinal barrier dysfunction (video abstract)

Dust mite allergens in the human GI tract: a potential contributor to intestinal barrier dysfunction (video abstract)

French version

http://unspod.unice.fr/video/4122-les-acariens-de-nouveaux-acteurs-dans-les-maladies-inflammatoires-intestinales-video-abstract/


To cite: Tulic MK, Vivinus-Nebot M, Rekima A et al. Presence of commensal house dust mite allergen in human gastrointestinal tract: a potential contributor to intestinal barrier dysfunction.

Background

Abnormal gut barrier function is the basis of gut inflammatory disease. It is known that house dust mite aero-allergens induce inflammation in respiratory mucosa. We have recently reported allergen from Dermatophagoides pteronyssinus to be present in rodent gut.

Objective

To examine whether Der p1 is present in human gut and to assess its effect on gut barrier function and inflammation.

Design

Colonic biopsies, gut fluid, serum and stool were collected from healthy adults during endoscopy. Der p1 was measured by ELISA. Effect of HDM was assessed on gut permeability, tight-junction and mucin expression, and cytokine production, in presence or absence of cysteine protease inhibitors or serine protease inhibitors. In vivo effect of HDM was examined in mice given oral HDM or protease-neutralised HDM. Role of HDM in low-grade inflammation was studied in patients with IBS.

Results

HDM Der p1 was detected in the human gut. In colonic biopsies from healthy patients, HDM increased epithelial permeability, reduced expression of tight-junction proteins and mucus barrier. These effects were associated with increased TNF-α and IL-10 production and were abolished by cysteine-protease inhibitor. HDM effects did not require Th2 immunity. Results were confirmed in vivo in mice. In patients with IBS, HDM further deteriorated gut barrier function, induced TNF-α but failed to induce IL-10 secretion.

Conclusions

HDM, a ubiquitous environmental factor, is present in the human gut where it directly affects gut function through its proteolytic activity. HDM may be an important trigger of gut dysfunction and warrants further investigation.

Read the full paper here: http://gut.bmj.com/content/65/5/757.long


French version

 


- Production :
Faculté de Médecine de l'Université Nice Sophia Antipolis
- Directeur de la publication
Pr Patrick BAQUÉ
- Directrice de la production
Isabelle CALLEA
- Réalisation:
Jean-Christophe VIALLE
- Scénario :
Emmanuelle DUCHEZ
Jean-Christophe VIALLE


- Traduction
Emmanuelle DUCHEZ
Valérie VERHASSELT
- Voix-off
Emmanuelle DUCHEZ
medecine.unice.fr

2015/2016
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